Figure 3
Impacts of BCG and/or Wnt/β-catenin signaling on ROS production in RAW264.7 cells. (A) RAW264.7 cells were infected with BCG at MOI of 10 for indicated time, then they were used for intracellular ROS measurement by a flow cytometry assay. A time-dependent ROS production was observed within 6 h. (B) RAW264.7 cells were infected with indicated doses of BCG for 6 h prior to be used for examination of intracellular ROS by a flow cytometry assay. A BCG dose-dependent ROS production was observed. (C) Impact of Wnt/β-catenin signaling on BCG-induced ROS production in RAW264.7 cells. RAW264.7 cells were infected with BCG at MOI of 10 for 6 h prior to be used for measuring intracellular ROS by a flow cytometry assay. An activation of Wnt/β-catenin signaling exhibited an ability to reduce BCG-induced ROS production. (D) Impact of Wnt/β-catenin signaling on oxidative stress in RAW264.7 cells. RAW264.7 cells were exposed to 500 μmol/L of H2O2 for 6 h before they were harvested for intracellular ROS measurement. The addition of Wnt3a showed a capacity to scavenge oxidative stressed-ROS accumulation.