Figure 4

ROS is involved in the BCG-induced necrosis in RAW264.7 cells. The effect of ROS in the necrosis of BCG-infected cells was verified using a ROS scavenger NAC. (A) RAW264.7 cells were treated with BCG, NAC, or their combination, then they were used for intracellular ROS measurement by a flow cytometry assay within 6 h. (B) Representatives of scatter dot plot of flow cytometric analysis for the necrosis cell fraction for RAW264.7 cells infected with BCG, NAC, or their combination. (C) NAC inhibits BCG-induced cell necrosis. RAW264.7 cells were infected with BCG at MOI of 10 for 24 h prior to be used for flow cytometric analysis. Quantitative results revealed that NAC could inhibit cell necrosis induced by BCG. Compared to a control-CM treated cells, *: p < 0.05; **: p < 0.01; compared to its corresponding BCG treated cells, #: p < 0.05; ##: p < 0.01. Data represented the mean ± SD from three independent triplicated experiments (N = 9).