Figure 5

Activation of Wnt/β-catenin signaling increases GSH concentration in RAW264.7 cells. RAW264.7 cells were treated with BCG, Wnt3a, DKK1, H₂O₂ or their combination as indicated, they were then used for determination of intracellular GSH levels by an ELISA for GSH. (A) Impact of Wnt/β-catenin signaling on GSH production of RAW264.7 cells infected with BCG at MOI of 10 for 6 h. An activation of Wnt signaling by addition of Wnt3a-CM could induce GSH generation; in contrast, overexpression of Wnt inhibitor DKK1 reduced GSH levels. (B) Impact of Wnt/β-catenin signaling on GSH production of RAW264.7 cells exposed to oxidative stress H₂O₂ (500 μmol/L) or LPS (100 ng/mL) stimulation for 6 h. The Wnt signaling could increase intracellular reduced GSH concentration in cells stressed by H₂O₂ and LPS. Compared to a control-CM treated cells, *: p < 0.05, **: p < 0.01. Control-CM treated cells compared to its corresponding Wnt3a-CM treated cells, #: p < 0.05; ##: p < 0.01. Data represented the mean ± SD from three independent triplicate experiments (N = 9).