Figure 2

Percentage of CD4 ⁺ CD25 ⁺ Foxp3 ⁺ Treg cells in blood, spleen and draining lymph nodes. (A) At 2wks postoperative, a single administration of either ATRA or TGF-β promoted the level of CD4⁺CD25⁺Foxp3⁺Treg cells in peripheral blood, spleen and draining lymph nodes, compared with those treated with a control solution. A mixed administration of ATRA and TGF-β further enhanced the level of Tregs. At 8wks postoperative, the level of CD4⁺CD25⁺Foxp3⁺ Treg cells in spleen and lymph nodes was increased in those with a single administration of either ATRA or TGF-β. Nevertheless, the synergistic effect was not found in those with a mixed treatment. Data are presented as the mean value ± standard error and are representative of at least three independent experiments. (B) Representative flow cytometric figures of Foxp3 and CD25 expression on CD3 + CD4 + cells from the blood, spleen and LN in recipient mice. * P < 0.05, ** P < 0.01, ***P < 0.001.