Table 1 Quantitative analysis of inflammatory cells, Foxp3+ cells and IL-17+ cells in grafts and conjunctiva in recipient mice
(cells/μm 2 ) | ATRA | TGF-β | ATRA + TGF-β | Control | Syngeneic | P value |
|---|---|---|---|---|---|---|
Inflammatory cells | ||||||
2w | 0 | 0 | 0 | 14.9 ± 18.72 | 0 | 0.132 |
4w | 496.1 ± 68.4 | 891.4 ± 80.9 | 93.2 ± 45.2 | 1842.5 ± 68.4 | 0 | <0.001 |
8w | 1356.0 ± 260.7 | 1843.2 ± 252.8 | 152.9 ± 52.9 | 1872.4 ± 106.3 | 29.8 ± 6.5 | <0.001 |
Foxp3+ cells | ||||||
2w | 21.9 ± 1.4 | 4.9 ± 0.6 | 16.7 ± 5.4 | 10.3 ± 2.7 | 25.6 ± 1.8 | <0.001 |
4w | 24.7 ± 0.6 | 29.4 ± 6.5 | 47.9 ± 2.2 | 22.2 ± 9.1 | 44.9 ± 6.7 | 0.004 |
8w | 32.5 ± 5.1 | 36.9 ± 4.3 | 52.6 ± 4.8 | 25.5 ± 6.2 | 36.4 ± 7.3 | 0.001 |
IL-17+ cells | ||||||
2w | 5.5 ± 1.0 | 8.5 ± 0.8 | 2.4 ± 0.4 | 8.1 ± 0.6 | 3.3 ± 1.2 | 0.017 |
4w | 3.5 ± 2.3 | 3.5 ± 0.2 | 5.1 ± 1.6 | 46.7 ± 12.4 | 5.1 ± 0.8 | <0.001 |
8w | 7.9 ± 1.7 | 8.7 ± 1.2 | 2.9 ± 1.3 | 50.0 ± 4.8 | 3.8 ± 1.1 | <0.001 |