Skip to main content
Figure 1 | BMC Immunology

Figure 1

From: Platelets convert peripheral blood circulating monocytes to regulatory cells via immunoglobulin G and activating-type Fcγ receptors

Figure 1

IgG-opsonized platelets augment IL-10 release but decrease proinflammatory cytokine release from monocytes via their direct contact. (A) A crude PBMC preparation was stimulated with LPS or poly(I:C) in the presence of anti-human CD61 mAb or isotype-matched control (cIg). The IL-10, IL-1β, and IL-12 p40 levels in the culture supernatants collected at different time points were determined by ELISA. (B) A crude PBMC preparation was stimulated with LPS in the presence of an anti-CD61 or anti-CD41 mAb or isotype control (cIg). The IL-10 and IL-12 levels in the culture supernatant after 24 h were determined by ELISA. Blank (−) indicates cells with no antibody or stimulator for monitoring spontaneous production of cytokines. (C) Transwell assaying of cytokine production. Sort-purified monocytes were stimulated with LPS in the presence or absence of platelets and an anti-CD61 mAb. The bottom chamber contained monocytes or was left blank, and the upper chamber contained platelets. Both chambers contained LPS and the anti-CD61 mAb. The IL-10 (left) and IL-1β (right) levels in the culture supernatant were measured after 24 h. *P < 0.05; **P < 0.01; n.s., not significant.

Back to article page