Figure 5
Differential T cell subpopulations in spinal cord of EAE mice treated with OGF or LDN. Differential cell counts of lymphocytes isolated from spinal cords isolated from mice immunized with MOG35–55 and treated from the time of induction with 10 mg/kg OGF (OGF), 0.1 mg/kg naltrexone (LDN), or 0.1 ml saline (saline); normal mice received saline at each injection point. Representative FACS profiles of T-cell populations obtained by fluorescence-activated cell sorting. Lymphocytes were isolated from spinal cord and stained with markers for CD4+, Th1, Th2, and Th17T cells. (A) Parameters for representative flow cytometry dot plots. (B) Representative flow cytometry dot plots for OGF, LDN, and saline treated mice 15 days after immunization with MOG35–55. (C) Histograms represent the percentage (± SEM) of electronically gated cells obtained from flow cytometry; samples were run in triplicate. Little or no Th2 cells were detected. Significantly different from saline-treated mice at p > 0.001 (***).