Fig. 1

Morphological changes in T cells following IS formation. a Left panel: An elongated-flattened CD4⁺ T cell before and after T cell-DC contact. TCRs were labelled with H57-597-Fab-Alexa 647 (red), and ICAM-1 was fused with EGFP (green). Right panel: Grey value profiles along the red and black lines in the BF image. The black line is before contact, and the red line is after contact. b A round-flattened CD4⁺ T cell before and after T cell-DC contact (left panel) and the grey value analysis (right panel). In (a) and (b), the last column in the left panel is 3D images of the contact surface which consisted of ICAM-1 (green) surrounding TCRs (red). The dotted white line depicts the contact boundary between the CD4⁺ T cells and the DCs. c A resting CD4⁺ T cell (left panel) at 0 min (top line) and 10 min (bottom line). The right panel depicts the grey value profiles along the red and black lines. Scale bar = 2 μm. d Comparison of the shape index of elongated and/or flattened CD4⁺ T cells and control CD4⁺ T cells before and after T cell-DC contact (round-flattened: 1.01 ± 0.10 vs 1.16 ± 0.18, elongated-flattened: 1.00 ± 0.10 vs 1.86 ± 0.53, control: 1.00 ± 0.10 vs 0.98 ± 0.12). In total, 25 cells were analysed in each group (the data are shown as the mean ± s.e.m.)