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Fig. 3 | BMC Immunology

Fig. 3

From: Morphological change of CD4+ T cell during contact with DC modulates T-cell activation by accumulation of F-actin in the immunology synapse

Fig. 3

Morphological changes correlated with the localisation of TCR signalling molecules in the IS. a-d Left panel: The localisations of TCR, ZAP-70, PLC-γ1, and PKC-θ (all in blue) were measured in a T cell becoming elongated-flattened (upper row) or round-flattened (bottom row) when IS formation. TCR (red) and ICAM-1 (green) were labelled to show the IS formation. The dotted white line depicts the contact boundary between the OT-II CD4+ T cells and the DCs. Scale bar = 2 μm. Right panel: A quantitative analysis was used to compare the MFIs of the T-cell signalling molecules in the IS of the round-flattened T cells and the elongated-flattened T cells (mean ± s.e.m., n=25, three independent experiments), ***P<0.001 (two-tailed Student’s t-test). Scale bar = 2 μm

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BMC Immunology

ISSN: 1471-2172

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