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Fig. 1 | BMC Immunology

Fig. 1

From: Negative feedback circuit for toll like receptor-8 activation in human embryonic Kidney 293 using outer membrane vesicle delivered bi-specific siRNA

Fig. 1

a Transport into outer membrane vesicle: In the presence of periplasmic signal (torA) gfp transportation to periplasm was significant (*P < 0.001) compared to construct which lacked torA signal (n = 10). b Fusion of lipid bilayer by diINF-7: The fusogenic activity was observed in presence of diINF-7 along with torA signal at pH 5.0(*P < 0.001) and 4.5(**P < 0.001). Also, the fusogenic activity was affected by presence or absence of torA signal peptide at pH 5.0(°P < 0.001) and 4.5(°°P < 0.001) but was independent of presence or absence of Ail/OmpA. c TLR8-HEK293 invasion assay: In presence of OmpA signal, the invasion was rapid as well at 30 minutes of assay the fluorometric output increased significantly (**P < 0.001). Values show the mean ± S.D (n = 10). The statistical analysis was performed by unpaired Student’s t test (*P < 0.001)

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BMC Immunology

ISSN: 1471-2172

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