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Fig. 2 | BMC Immunology

Fig. 2

From: Negative feedback circuit for toll like receptor-8 activation in human embryonic Kidney 293 using outer membrane vesicle delivered bi-specific siRNA

Fig. 2

a Cell death as marker of transport to cytoplasm: The cell viability decreased in presence of saporin which peaked in 48 h (**P < 0.001). The construct lacking any of Ail (°P < 0.001), diINF-7 (°P < 0.001), torA (°P < 0.001) and OmpA (°P < 0.001) failed to decrease the cell viability significantly in presence of saporin construct. b The presence of p19 was essential for binding of siRNA (°P < 0.001). The addition or deletion of p19  (°P < 0.001)/siRNA (*P < 0.001)/diINF-7 (•P < 0.001) had significant effect in INFβ production as well MyD88 transcript abundance. c pH dependent dissociation was observed and siRNA was free from p19 complex (*P < 0.001). Values show the mean ± S.D (n = 10). The statistical analysis was performed by unpaired Student’s t test (*P < 0.001)

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BMC Immunology

ISSN: 1471-2172

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