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Fig. 4 | BMC Immunology

Fig. 4

From: IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus

Fig. 4

IFNγ+ Treg induced in-vitro in the presence of both rIFNγ and PMA/Ionomycin. a PBL of 5 healthy individuals were stimulated for 72 h using PMA/Ionomycin in the presence of 500, 1000, 5000 ng/ml or without rIFNγ. PMA/Ionomycin-stimulated cultures without rIFNγ were compared with those stimulated in the presence of rIFNγ. b, c The same assay was performed using cell cultures with enriched CD4+CD25+ or enriched CD4+CD25- PBL. The data suggest that CD4+CD25+ PBL are rather resistant to the effect of rIFNγ. In contrast, CD4+CD25- enriched PBL showed increasing total Treg with both Helios+ Treg switching from IFNγ- to IFNγ+ and increasing IFNγ+Helios- Treg indicating that rIFNγ in combination with a polyclonal stimulus induces activation of nTreg as well as induction of aTreg. Data are given as mean ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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BMC Immunology

ISSN: 1471-2172

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