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Fig. 5 | BMC Immunology

Fig. 5

From: IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus

Fig. 5

Kinetics of Treg subsets induced in-vitro from CD4+CD25+ or CD4+CD25- PBL preparations after elimination of the stimulus. Unseparated as well as enriched CD4+CD25+ and CD4+CD25- cell preparations were stimulated with PMA/Ionomycin for 6 h and Treg subsets were determined. Mitogen was removed by several washes of the cell culture and Treg subsets were determined again immediately after the washes at 7 h and at 24, 48, 72 and 96 h after initiation of the cell culture. Assays were performed with PBL from 3 healthy volunteers. The measurement 96 h after initiation of the cell culture was performed with only one cell donor. CD4+CD25+Foxp3+CD127- Treg increased after removal of the stimulus. The strong increase of CD4+CD25+Foxp3+CD127- Treg was also observed when instead of unseparated PBL CD4+CD25- enriched PBL fractions were used. In contrast, CD4+CD25+ enriched PBL fractions showed only an increase of Helios-IFNγ+ Treg. Our data do not support the hypothesis that removal of the stimulus results in a significant decrease of IFNγ- as well as IFNγ+ CD4+CD25+Foxp3+CD127- Treg due to loss of Foxp3 expression. Data are given as mean ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001

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BMC Immunology

ISSN: 1471-2172

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