Skip to main content
Fig. 6 | BMC Immunology

Fig. 6

From: IFNγ+ Treg in-vivo and in-vitro represent both activated nTreg and peripherally induced aTreg and remain phenotypically stable in-vitro after removal of the stimulus

Fig. 6

Treg subsets and Foxp3 TSDR DNA methylation analysis in enriched CD4+CD25+CD127-IFNγ+PBL preparations. a CD4+CD25+CD127-IFNγ+ Treg were separated from CD4+CD25+CD127-IFNγ- Treg using PBL from 5 different healthy volunteers. CD4+CD25+Foxp3+CD127- co-expressing Helios-IFNγ+ were significantly enriched in the IFNγ+ Treg preparation whereas CD95+CD178- Treg were depleted compared to IFNγ- Treg preparations. Data are given as mean ± SEM. *p ≤ 0.05. b-d Foxp3 TSDR DNA methylation analysis of enriched CD4+CD25+CD127-IFNγ+ (b, c) and enriched CD4+CD25+CD127-IFNγ- (d, e) Treg fractions from 12 healthy individuals (samples 1 – 12). Figure b and d and Fig. c and e correspond with each other showing test results of the same individuals. In addition, melt curves of the standards with 0, 10, 50 and 100 % methylated Foxp3 TSDR DNA are depicted in each diagram. 4 of 6 IFNγ+ Treg preparations in Fig. 6b, 3 of 6 IFNγ+ Treg preparations in (c), 3 of 6 IFNγ- Treg preparations in (d) and 5 of 6 IFNγ- Treg preparations in (e) showed a Foxp3 TSDR methylation of <50 % representing mainly demethylated Treg with stable Foxp3 expression

Back to article page

BMC Immunology

ISSN: 1471-2172

Contact us