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Fig. 6 | BMC Immunology

Fig. 6

From: Immune cell subsets and their gene expression profiles from human PBMC isolated by Vacutainer Cell Preparation Tube (CPT™) and standard density gradient

Fig. 6

Yield and quality of RNA recovered from total PBMC and their individual immune cell subsets did not significantly differ between PBMC isolated by Ficoll and CPT methods. a The mean amount of RNA per cell and (b) the mean RIN of RNA preparations extracted from total PBMC and their individual immune cell subsets derived from PBMC prepared by Ficoll and CPT methods from 6 health donors. There were no significant differences (P < 0.05) in the amount of RNA obtained and in the quality of RNA, as measured by RIN, between the two methods of PBMC isolation. Error bars indicate standard error of the mean. RIN, RNA integrity number

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BMC Immunology

ISSN: 1471-2172

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