Sequence conservation analysis and in silico human leukocyte antigen-peptide binding predictions for the Mtb72F and M72 tuberculosis candidate vaccine antigens

Table 2 MHC class II-binding peptide predictions for common DRB1 alleles in high-TB burden regions

Region/Alleles assessed (N)	Protein	Predicted epitopes (N)		DRB1 alleles without predicted epitope
Region/Alleles assessed (N)		Total	Average/allele	N	Allele
Overall	Mtb32A	8792	56	5	03:02, 04:07, 14:10, 14:14, *14:44
(N = 158)	Mtb39A	14675	93	2	03:02, 16:04
	Mtb72F	22065	140	2	03:02, 16:04^a
China	Mtb32A	5873	40	5	03:02, 04:07, 14:10, 14:14, *14:44
(N = 146)	Mtb39A	12794	88	2	03:02, 16:04
	Mtb72F	19109	131	2	03:02, 16:04^a
N. India	Mtb32A	2595	55	2	03:02, 04:07
(N = 47)	Mtb39A	4516	96	1	*03:02
	Mtb72F	6733	143	1	*03:02^a
S. India	Mtb32A	1087	43	0
(N = 25)	Mtb39A	1993	80	1	*16:04
	Mtb72F	2868	115	1	*16:04^a
N.E. India	Mtb32A	2155	51	2	03:02, 04:07
(N = 42)	Mtb39A	3517	84	1	*03:02
	Mtb72F	5298	126	1	*03:02^a
India (Total)	Mtb32A	2807	37	2	03:02, 04:07
(N = 76)	Mtb39A	4634	61	2	03:02, 16:04
	Mtb72F	6876	90	2	03:02, 16:04^a
S.S. Africa ^b	Mtb32A	4447	67	1	*03:02
(N = 66)	Mtb39A	6977	106	1	*03:02
	Mtb72F	10737	163	1	*03:02^a

Prediction results represent the sum of the weak and strong binders. ^aAllele frequencies in the populations assessed are included in Additional file 2. ^bS.S. Africa, Sub-Saharan Africa, native population only

ISSN: 1471-2172