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Fig. 1 | BMC Immunology

Fig. 1

From: β-Catenin is required for the differentiation of iNKT2 and iNKT17 cells that augment IL-25-dependent lung inflammation

Fig. 1

β-CAT-Tg mice show an exacerbated response to IL-25-dependent AHR induction. β-CAT-Tg, β-CAT-cKO and control mice were sensitized with OVA, treated with IL-25 or PBS and finally challenged with OVA to induce airway hyperreactivity (AHR). a Histological analysis of lung tissues with hematoxylin and eosin (H&E) and periodic acid Schiff (PAS) staining. Representative control and β-CAT-Tg sections are shown. H&E bars, 100 μm; PAS bars, 100 μm. Graph shows percentage of PAS-positive cells per total number of cells in the epithelium of the airway, counting 5–7 airways per lung section (mean ± sem). b Total cellularity (Total) and cell numbers of eosinophils (Eos), alveolar macrophages (alv MO), neutrophils (Neu) and lypmhocytes (Lym) present in bronchoalveolar lavage (BAL) fluid (n = 5, mean ± sem) from control and β-CAT-Tg (top) and control and β-CAT-cKO (bottom). c IL-4+, IL-13+ and IL-17A+ cell percentages of lung iNKT cells after IL-25-dependent AHR induction from control and β-CAT-Tg (top) and control and β-CAT-cKO (bottom) (n = 5, mean ± sem). AHR experiment was performed twice, with 3–5 mice per group in each independent experiment. * P < .05; ** P < .01

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