Fig. 5

Enhanced generation of β-catenin-dependent iNKT2 and iNKT17 cells is cell autonomous. a Flow cytometry of a bone-marrow chimera experiment analyzed after 7–8 weeks, in which CD45.1+ recipient C57BL/6.SJL mice were irradiated and 2 million cells isolated from bone marrow were i.v. injected from CD45.2+ donors, either control or β-CAT-Tg mice. Top, total iNKT cells gated on CD45.2+ cells. Middle, iNKT1, iNKT2 and iNKT17 cells within the iNKT populations. Bottom, number of total iNKT, iNKT1, iNKT2 and iNKT17 cells from donor control and donor β-CAT-Tg cells (n ≥ 8, mean ± sem). b Flow cytometry of CD45.1 and CD45.2 on thymic cells of mixed bone-marrow chimeras containing CD45.1+ recipient C57BL/6.SJL cells, CD45.1 + CD45.2+ donor control cells and CD45.2+ donor β-CAT-Tg cells. iNKT cells from both donor cells (top) and iNKT1, iNKT2 and iNKT17 cells within the iNKT populations (Middle). Representative data out of three mice analyzed. Bottom, number of total iNKT, iNKT1, iNKT2 and iNKT17 cells from donor control and donor β-CAT-Tg cells (n = 3, mean ± sem). Mice were analyzed 7 weeks after mixed bone-marrow chimeras were performed. * P < .05; ** P < .01; *** P < .001