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Fig. 5 | BMC Immunology

Fig. 5

From: In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells

Fig. 5

Cytokine production in NK, CIK, and γδ T cells. a Intracellular staining of IFN-γ and IL-2 in NK, CIK, and γδ T cells. Intracellular levels of IL-2 and IFN-γ were measured as described for perforin and granzyme B detection. b Extracellular cytokine production of NK, CIK, and γδ T cells. The culture supernatants were collected and analyzed by ELISA for IFN-γ, IL-2, IL-4, IL-6, and IL-10. Results are expressed as the mean ± SD, n = 20. * p < 0.05, ** 0.001 < p < 0.01, *** p < 0.001, ns p > 0.05

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