Skip to main content
Fig. 5 | BMC Immunology

Fig. 5

From: Mycobacterium tuberculosis secretory proteins downregulate T cell activation by interfering with proximal and downstream T cell signalling events

Fig. 5

M. tuberculosis antigens modulate TCR- and TCR/CD28-induced DNA binding of NFAT to the promoter of IL-2 cytokine in PPD+ve healthy individuals and pulmonary TB patients. (a) Peripheral blood mononuclear cells (PBMCs) from 11 PPD+ve healthy donors and 10 pulmonary TB patients were stimulated with M. tuberculosis antigens for 4 h. Cells were then activated with anti CD3 and anti CD28 antibodies (as mentioned in methodology). Nuclear lysates were prepared and binding affinity of NFAT was determined by EMSA. One representative example of each study case is shown where Lane 1- Control, Lane 2- anti CD3 stimulated, Lane 3- anti CD3 + anti CD28 stimulated, Lane 4- anti CD3 + anti CD28 stimulated in presence of Ag85A, Lane 5- anti CD3 + anti CD28 stimulated in presence of ESAT-6, Lane 6- anti CD3 + anti CD28 stimulated in presence of PPD and Lane 7-anti CD3 + anti CD28 stimulated in presence of H37Rv. (b) Densitometry was performed, Histograms represent mean band intensities. *P < .05,** P <0.005; *** P <0.005 by the Mann–Whitney U test

Back to article page