Fig. 5

Involvement of Dcir1 in the oxidative burst of neutrophils in response to LPS and GM-CSF. a Neutrophils sorted from Dcir1 ^−/− and WT bone marrow were stimulated with 100 ng/ml LPS for 6 h in the presence of DHR-123 for the last 30 min of culture, and conversion to rhodamine-123 (Rho-123) was assessed by flow cytometry. b ROS production of neutrophils was analyzed as in (a) after stimulation with 10 ng/ml GM-CSF for 6 h. Results are representative of two independent experiments (n = 4). ****P < 0.001. Data are expressed as the mean ± SEM