Fig. 2

CD5⁺ B cells in 12 week-old dnRAG1 and DTG mice support inducible IL10 expression in vitro, but IL10 competence is CD1d-independent. a-b Splenocytes from 12 week-old wild-type, dnRAG1, Eμ-TCL1, and DTG mice on a Cd1d ^+/+ (a) or Cd1d ^del/del (b) background were incubated with monensin alone or LPS + PIM for 4 h. After permeabilization, the cells were stained with IL-10-specific or isotype-control monoclonal antibody (Ab) as indicated. Gated CD19⁺ B cells were analyzed for CD5 and IL10 expression. The percentage of cells in each quadrant is shown for representative animals. c The mean percentage of total CD5⁺ B cells staining positive for IL10 for n = 4 mice/genotype is plotted in bar graph format. Error bars represent the SEM. Statistically significant (p < 0.05) differences between groups of WT and transgenic animals with the same Cd1d genotype are indicated (a, vs dnRAG1; b, vs Eμ-TCL1; c, vs DTG). No significant differences between Cd1d ^+/+ and Cd1d ^del/del animals with the same transgenes were detected