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Fig. 4 | BMC Immunology

Fig. 4

From: Cd1d regulates B cell development but not B cell accumulation and IL10 production in mice with pathologic CD5+ B cell expansion

Fig. 4

Progression through the transitional stages of B cell development is impaired in Cd1d del/del mice. a Gated splenic CD19+CD5 B cells from 12 week-old or 36 week-old WT, dnRAG1, Eμ-TCL1 and DTG mice on a Cd1d +/+ (+/+) or Cd1d del/del (del/del) background were initially segregated into transitional (trans.) and mature B cell subsets based on differential expression of AA4.1 and B220. Transitional (AA4.1+B220hi) and mature (AA4.1B220hi) B cell subsets were further separated into T1-T3, marginal zone (MZ) and follicular mature (FM) populations based on differential expression of IgM, CD21, and CD23 using the gating strategy found at the bottom of the figure. The percentage of cells within the identified gates is shown for representative animals. Data collected from n = 4–6 animals/genotype were used to calculate the absolute number of cells in each population, and are summarized in Additional file 2: Table S1 and Additional file 4: Table S2. b Values for the mean absolute number of total transitional B cells (B220hiAA4.1+CD5) and for each transitional B cell subset (T1-T3) are plotted in bar graph format for the 12 week time point in (a). Error bars represent the standard error of the mean. Statistically significant differences (p < 0.05) between groups of Cd1d +/+ or Cd1d del/del mice with the same transgenic background are identified by an asterisk

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