Fig. 5

Loss of CD1d expression in 36 week-old DTG mice is associated with expansion of splenic T cells, particularly CD8⁺ T cells. a Gated splenic CD3⁺B220⁻ lymphocytes were analyzed for CD4 and CD8 expression. The percentage of cells within the identified gates or quadrants is shown for representative animals. Note that in DTG mice, the B220⁻ gate does not exclude all CD5⁺ B cells, which are highly abundant and express variably low levels of B220 in these animals. Data collected from n = 4–6 animals/genotype were used to calculate the absolute number of cells in each population, and are summarized in Additional file 4: Table S2. b Values for the mean absolute number of CD4⁺ and CD8⁺ T cells determined from (a) are plotted in bar graph format. Error bars represent the standard error of the mean. Statistically significant differences (p < 0.05) between groups of Cd1d ^+/+ or Cd1d ^del/del mice with the same transgenic background are identified by an asterisk