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Fig. 5 | BMC Immunology

Fig. 5

From: PHF11 expression and cellular distribution is regulated by the Toll-Like Receptor 3 Ligand Polyinosinic:Polycytidylic Acid in HaCaT keratinocytes

Fig. 5

siRNA knock-down of PHF11 decreases nuclear PHF11 in poly(I:C) treated cells. Cells were transfected with control (siCon) or PHF11-specific (siPHF11) siRNA (day 1) and then treated with 1 μg/ml of poly(I:C) (day 3) for 24 h. a. Image of transfected cells showing an absence of PHF11 in cell nuclei. Arrows indicate the location of nuclei with reduced PHF11. Green: PHF11; blue: nuclei. Scale bar 10 μM. b. Western blot showing knock-down of PHF11 in nuclear lysates in the absence and presence of PHF11. Shown are the results for control (c) and two different PHF11-specific siRNAs either singly (1 & 5) or in combination (1/5)

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