Fig. 7

Knock-down of PHF11 expression increases IL-8 expression immediately after treatment with poly(I:C). a Quantitative real-time PCR analysis of IL8 RNA in cells transfected with control (con) or PHF11-specific (PHF11) siRNA. Results are normalized to untreated cells transfected with control siRNA. b Immunohistochemistry of IL-8 synthesis. Red: IL-8; Blue: nuclei. Quantification of IL-8 positive nuclei is shown on the right. Asterisks indicate significant difference relative to control (p < 0.05, Mann–Whitney U-test). c ELISA quantification of IL-8 secretion from siRNA-transfected HaCaT cells. Cells were transfected with control (siRNA_Con) or PHF11-specific siRNA (siRNA_1, _5, and a combination of 1 & 5) and then treated with poly(IC) (1 or 10 μg/ml) for 24 h prior to assaying for secreted IL-8. Results represent 4 independent experiments; asterisks indicate significant differences (p < 0.05, Kruskal-Wallis test). Scale bar 20 μM