Fig. 4

Oil granuloma development after pristane injection. Oil granulomas were formed in both groups of animals, though their organizations differed between them. After 60 days of pristane injection, oil granulomas can be macroscopically observed attached to the mesentery (white arrows), both in WT (a) and gal-3^−/− mice. b WT mice oil granulomas (c) were organized in cortical and medullary-like regions (dotted line) and were rich in blood vessels (e and red circles in c), while gal-3^−/− mice oil granulomas presented random distribution of cells (d) and few blood vessels (red circles in d), some of them surrounded by inflammatory cells (arrows in f). CD31⁺ staining for blood vessels confirmed the presence of these structures both in WT and gal-3^−/− mice (g and h, respectivelly). The staining intensity of CD31 was quantified in WT and gal-3^−/− mice (i). Data are representative of five animals of each group