Fig. 1

Experimental setup of the pSILAC method. a Cells grown in L medium were transfected with anti-miR-130a-LNA or scrambled-LNA (mock-transfection) and transferred to M or H SILAC-medium, respectively. After 48 h (32Dcl3 miR-130a clone, doubling time ~18–20 h) or 72 h (Kasumi-1, doubling time ~40 h) of pulse labelling, cells were washed, combined 1:1, and lysed. Samples were prepared for and analysed by LC-MS/MS, producing peptide peaks as shown (b). The light peptides were disregarded while the M and H peptides were compared, generating M/H ratios for further analysis