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Table 1 Use of MSCs to promote engraftment in murine models

From: Mesenchymal stromal cells to modulate immune reconstitution early post-hematopoietic cell transplantation

HSC source Model Conditioning MSC source MSC dose Outcome Ref
Human UCB CD34+cells NOD/SCID mice 3.5 Gy Human fetal lung 1 × 106 3-4 fold increased donor CD45+ (lymphoid & myeloid, not B cell) 21
Human UCB CD34+cells NOD/SCID mice 3.5 Gy Fetal lung, liver, or BM or adult BM 1 × 106 Fetal lung & BM & adult BM MSCs increased engraftment of donor CD45+ in BM, PB, or spleen 22
Human UCB CD34+cells (single vs double) NOD/SCID mice 3.25 Gy Human placenta 4 × 104 Increased engraftment (total & CD34+), no effect on CD19+ Decreased single cord dominance 13
Human UCB CD34+cells (single vs double) NOD/SCID mice 3.5 Gy Human BM NR Increased engraftment, decreased single cord dominance 23
Human PB CD133+cells NOD/SCID mice 3 Gy Human BM; CD271+ & PA-MSC 1 × 105(1:1; HSC:MSC) 7 × 105 (1:8) Increased engraftment (CD45+, CD33+, CD19+), no effect on CD41a+, CD3+, CD56+ Increased engraftment (CD271+ > PA-MSC), comparable increase CD33+ & CD41a+, CD271+ increase lymphoid & decrease NK cells 24
Human UCB CD34+cells NOD/SCID mice 3.5 Gy Human BM (STRO-1+ or STRO-1) 1 × 106 STRO-1 MSC increased engraftment of donor CD45+ in BM, PB, or spleen more than STRO-1+, STRO-1+ MSC improved homing to recipient tissue 25
C57BL/6 or BALB/b TCD BM cells BALB/c or C57BL6/-Ly-5.1 mice 5-6 Gy Syngeneic, allogeneic, or 3rd party BM 0.25 × 106; days 0, 4, 7, 10, 14 Syngenic MSCs increase engraftment, donor MSCs increase rejection, 3rd party MSCs no effect 14
Human PB CD34+cells NOD/SCID mice 3 Gy Autologous (PB) or allogeneic (BM), human 1 × 106 Increased donor CD45+ with either MSC source, allogeneic MSCs increased myeloid engraftment and megakaryocytopoiesis 15
  1. MSCs indicates mesenchymal stromal cells, HSC hematopoietic stem cell, UCB umbilical cord blood, NOD/SCID nonobese diabetic/severe combined immunodeficiency, Gy gray, BM bone marrow, NR not reported, TCD T cell depleted, PB peripheral blood, PA-MSC plastic-adherent MSC, NK natural killer