Fig. 4

Transient gene silencing reduces SIGIRR expression in T24 and 5637 cells. Cells were transfected with SIGIRR and negative control siRNA in transfection reagents. Efficiencies of SIGIRR siRNA for target gene expression knock-down in cells were evaluated by real-time PCR (a) and Western blot (b). The mRNA levels of SIGIRR expression in each of the samples were normalized by GAPDH and compared to that of the negative control siRNA, *p < 0.05 versus negative control siRNA