Fig. 5

SIGIRR gene silencing augmented inflammatory response and signaling upon LPS stimulation. a Increased response in SIGIRR gene silencing cells upon LPS stimulation. SIGIRR and negative control siRNA transfected cells were exposed to 100 ng/ml LPS, cell culture supernatants were collected after 6 h, levels of IL-6 and IL-8 were quantified by ELISA. Data represent the means ± SEM from three independent experiments, *p < 0.05 versus negative control siRNA. b Increased signaling in SIGIRR gene silencing cells upon LPS stimulation. SIGIRR and negative control siRNA transfected cells were stimulated with 100 ng/ml LPS for the indicated periods. Cells lysates were blotted with the indicated antibodies