Fig. 5From: Dectin-2-dependent host defense in mice infected with serotype 3 Streptococcus pneumoniae Cellular source of IFN-γ production in the lungs after pneumococcal infection. WT mice were infected with S. pneumoniae. The lung leukocytes prepared at 12 h post-infection were stained with various mAbs to identify populations of myeloid cells and lymphocytes, and intracellular IFN-γ expression was analyzed in each population using flow cytometry. Cut-off lines were determined on the histograms stained with isotype-matched IgG. Ex Mφ, exudate macrophages; Alv Mφ, alveolar macrophages; Neu, neutrophils; IgG, isotype-matched IgGBack to article page