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Fig. 8 | BMC Immunology

Fig. 8

From: Dectin-2-dependent host defense in mice infected with serotype 3 Streptococcus pneumoniae

Fig. 8

Dectin-2-dependent IL-12p40 production by BM-DCs in the ConA-bound fraction of S. pneumoniae culture supernatant. a BM-DCs derived from WT mice were stimulated with the ConA-Sepharose4B-treated or sham-treated supernatant from S. pneumoniae or mannan for 24 h. b BM-DCs from WT mice (open columns) or Dectin-2KO mice (solid columns) were cultured with the ConA-Sepharose4B-bound fraction of S. pneumoniae culture supernatants, LPS (1 μg/ml) or mannan (3 mg/ml) for 24 h. The IL-12p40 concentration in the culture supernatants was measured by ELISA. Each column shows the mean ± SD of triplicate culture. Similar results were obtained in three independent experiments. Sup-sham, sham-treated supernatants; Sup-tx, ConA-Sepharose4B-treated supernatants; Mannan-sham, sham-treated mannan; Mannan-tx, ConA-Sepharose4B-treated mannan; ConA-bound Fr, CpnA-Sepharose4B-bound fraction. * p < 0.05

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