TY - JOUR AU - Yang, Jun AU - Diaz, Norma AU - Adelsberger, Joseph AU - Zhou, Xueyuan AU - Stevens, Randy AU - Rupert, Adam AU - Metcalf, Julia A. AU - Baseler, Mike AU - Barbon, Christine AU - Imamichi, Tomozumi AU - Lempicki, Richard AU - Cosentino, Louis M. PY - 2016 DA - 2016/03/15 TI - The effects of storage temperature on PBMC gene expression JO - BMC Immunology SP - 6 VL - 17 IS - 1 AB - Cryopreservation of peripheral blood mononuclear cells (PBMCs) is a common and essential practice in conducting research. There are different reports in the literature as to whether cryopreserved PBMCs need to only be stored ≤ −150 °C or can be stored for a specified time at −80 °C. Therefore, we performed gene expression analysis on cryopreserved PBMCs stored at both temperatures for 14 months and PBMCs that underwent temperature cycling 104 times between these 2 storage temperatures. Real-time RT-PCR was performed to confirm the involvement of specific genes associated with identified cellular pathways. All cryopreserved/stored samples were compared to freshly isolated PBMCs and between storage conditions. SN - 1471-2172 UR - https://doi.org/10.1186/s12865-016-0144-1 DO - 10.1186/s12865-016-0144-1 ID - Yang2016 ER -