The effects of storage temperature on PBMC gene expression

BMC Immunology

Table 3 Comparison of microarray data to RT-PCR data for 13 genes that align with biological pathways

Associated biological pathway	Tested gene^a	Microarray data (Log2[FC])		RT-PCR data (ΔΔCT)
Associated biological pathway	Tested gene^a	Stored ≤ −150 °C to Fresh	Stored −80 °C to Fresh	Stored ≤ −150 °C to Fresh	Stored −80 °C to Fresh
Interferon Signature Genes	MX1	−0.97	−0.88	−1.93	−1.17
	MX2	−0.74	−0.50	−1.60	−0.91
	IFNG	2.21	3.22	2.50	4.38
	IFI16	−1.81	−1.47	−2.93	−1.77
	OAS2	−1.79	−1.91	−3.29	−2.49
Cell Death-Related Genes	GADD45B	2.10	2.49	1.48	2.61
Cell Death-Related Genes	FOSL2	3.14	2.98	2.19	2.49
Immune Response Genes	IL1B	4.39	5.19	5.91	7.26
	TNF	1.39	2.00	1.56	2.94
	TNFSF10	−3.23	−2.72	−3.92	−2.88
MAPK Genes	NFKB1	1.63	1.32	1.13	1.21
	MAP3KB	3.07	3.20	1.93	2.05
	DUSP4	4.59	4.10	5.85	6.07

^a Abbreviations: MX1 (myxovirus [influenza virus] resistance 1, interferon-inducible protein p78 [mouse], MX2 (myxovirus [influenza virus] resistance 2 [mouse], IFNG (interferon, gamma), IFI16 (interferon, gamma-inducible protein 16), OAS2 (2′–5′-oligoadenylate synthetase 2, 69/71 kDa), GADD45B (Growth arrest and DNA-damage-inducible, beta), FOSL2 (FOS-like antigen 2), IL1B (interleukin 1, beta), TNF(tumor necrosis factor), TNFSF10 (tumor necrosis factor (ligand) superfamily, member 10), NFKB1 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 1), MAP3K8 (mitogen-activated protein kinase kinase kinase 8), and DUSP4 (dual specificity phosphatase 4)

ISSN: 1471-2172