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Fig. 1 | BMC Immunology

Fig. 1

From: Methamphetamine abuse affects gene expression in brain-derived microglia of SIV-infected macaques to enhance inflammation and promote virus targets

Fig. 1

Characterization of cell subpopulations in the brain-derived cell isolates. Percoll-isolated cells from the brain of rhesus macaques were utilized in this study. These cells were characterized based on the expression of surface markers of cell subpopulation, and analyzed flow cytometry. a Cell gating strategies. The cells were gated based on the expression of CD11b (myeloid cell marker) and CD45LCA (peripheral cell marker), where CD11bint CD45LCA- cells are microglia, CD11b + CD45LCA+ cells are macrophages and CD11b-CD45LCA+ cells are mostly lymphocytes. The identity of microglia and macrophages was further confirmed by the CD14 and CD16 expression patterns. b Percentage of Lymphoid cells. The CD11b- population was characterized based on the expression of lymphocyte markers CD3, CD4 and CD8, and the relative differences in the percentage of lymphocyte subsets between groups is shown. *p ≤ 0.05 compared to uninfected controls. c Myeloid cell subset characterization. Myeloid cell subsets were differentially analyzed based on the expression of inflammatory status surface markers, CX3CR1, CD44v6, CCR5, CCR2 and CD80. *p ≤ 0.05 compared to uninfected controls. Microglia activation: Immunohistochemical detection of Iba-1 microglial marker in brains from animals that were (d) Controls, (e) SIV-infected, (f) Meth-treated, (g) SIV-infected and Meth-treated. Sections from the frontal cortex were examined for the frequency and distribution of Iba-1-expressing cells. The panels show one representative animal per group

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BMC Immunology

ISSN: 1471-2172

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