Fig. 5
Expression of paired Trem genes in BMDMs. a. Dose-dependent expression of paired Trem genes in response to polyI:C stimulation in BMDMs. Day 7-harvested BMDMs (2 × 105 cells /well) were incubated with polyI:C for 12 h, the concentration of polyI:C were 10 μg/ml, 20 μg/ml and 50 μg/ml. b Time-dependent expression of paired Trem genes in response to polyI:C stimulation in BMDMs. Day 7-harvested BMDMs (2 × 105 cells /well) were incubated with polyI:C by the concentration of 20 μg/ml. The incubation times with polyI:C were 4, 8 and 12 h. Relative expression levels of Trem genes were evaluated by qPCR. The data shown are representative of at least two independent experiments. Data are means ± SD of three independent samples. *p < 0.05. **p < 0.01