Fig. 6

Expression of paired Trem genes in tissues and splenic DC subsets. a Tissue distributions of paired Trem transcripts in adult healthy mice. N.D., not detected. b Paired Trem expression after polyI:C stimulation in spleen. Wild-type mice were intraperitoneally injected with polyI:C (200 μg/ml). After 3 h, total RNA was isolated from spleen in each mouse. c Treml6 and Trem4 expression after polyI:C stimulation in splenic cDCs and pDCs. Wild-type mice were intraperitoneally injected with polyI:C (200 μg/ml). After 3 h, total RNA was isolated from FACS-sorted cDCs and pDCs. In these experiments, mRNA expressions of paired Trem genes were measured by qPCR and their expression levels were normalized to that of Gapdh mRNA. Data are representative of three independent experiments. The data shown are representative of at least two independent experiments. Data are means ± SD of three independent samples. *p < 0.05. **p < 0.01