Fig. 1

Anaphylactic responses in mice. Anaphylactic responses in mice after intragastric (i.g.) gavages with Lupex, Lupex + cholera toxin (CT), Lupex + CT + trypsinised Cry1Ab (trypCry1Ab), Lupex + trypCry1Ab, and HBSS (control) on days 0, 1, 2, 7, 21, and 28, followed by an intraperitoneal (i.p.) challenge with Lupex on day 35. Rectal temperature (a) was measured at 0, 15 and 30 min after challenge, and anaphylactic score (b) were determined continuously during those 30 min. Mouse mast cell protease-1 (MCPT-1) levels (c), a marker for intestinal anaphylaxis, was measured in serum collected after challenge (day 35). Temperatures are presented as group medians over time, while anaphylactic scores and serum MCPT-1 concentrations are presented for each individual animal (dots) and as the group median (line). Asterisk (*) denote groups that are significantly different (p < 0.05) from the Lupex group. The dotted line denotes the upper detection limit of the MCPT-1 assay