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Fig. 5 | BMC Immunology

Fig. 5

From: Inhibition of Notch 1 receptor influenced the differentiation of Lin-CD45RA-dendritic cell precursors within ovarian carcinoma microenvironment

Fig. 5

a Flow cytometry analysis showed the differentiation of Lin-CD45RA-DC precursors without SKOV3 culture supernatants (n = 3, mean ± SD, %). shRNA mediated Notch 1 knock down significantly differentiated more mDCs(p = 0.001), but generated less pDCs (p = 0.007). GSI mediated Notch 1 knock down significantly differentiated less pDCs (p = 0.015), but had no effect on mDCs (p = 0.45). b Representative flow histograms of DCs subtypes obtained in different culture conditions using a mAb mixture containing HLA-DR, CD11c, and CD123 mAb. The cells were identified on the basis of their surface expression of HLA-DR, and then DCs were further analyzed by CD11C and CD123 expression within the HLA-DR+ gate. mDCs and pDCs were defined as HLA-DR + CD11c + CD123- and HLA-DR + CD11c-CD123 + 

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