Fig. 1
From: Metallothionein regulates intracellular zinc signaling during CD4+ T cell activation
[Zn2+]i and MT expression are regulated during CD4+ T helper cell differentiation and affected by IL-27. Mononuclear cells were isolated from spleens of C57BL/6 mice (n = 7) and stimulated for 6 days with anti-CD3 and anti-CD28 antibodies in the presence of IL-27 or without cytokines to induce a Tr1 or Th0 phenotype of CD4+ T cells, respectively. a, b CD25 expression, (c, d) intracellular [Zn2+] and (e, f) MT expression in the CD4+ T cell population were measured at 24-h intervals for 6 days. Histograms are representative of one Tr1 sample (solid line) and one Th0 sample (dotted line). g Mononuclear splenocytes from metallothionein knockout mice (MT-/-) (n = 11) or congenic wildtype controls (MT+/+) (n = 11) were cultured under Tr1 inducing conditions for 6 days and then rested for an additional 2 days. Intracellular [Zn2+] was measured on Day 0, Day 6, and Day 8 which corresponded to naïve, lymphoblast and effector stages of CD4+ T cell development, respectively. Error bars indicate standard deviation. *p < .05