Fig. 4
From: Metallothionein regulates intracellular zinc signaling during CD4+ T cell activation
Metallothionein gene dose affects intracellular [Zn2+] in Tr1 cells following stimulation through the T cell receptor. Tr1 cells from metallothionein knockout (MT-/-, n = 4) or congentic wildtype control mice (MT+/+, n = 4) were loaded with CM-H2DCFDA and stimulated with anti-CD3 [10 μg/mL] cross-linked with anti-hamster IgG [10 μg/mL] for 0–120 min. a The fold change in CM-H2DCF fluorescence or (b) intracellular [Zn2+] in stimulated Tr1 cells compared with unstimulated control cells for each strain at 30, 60 and 120 min is shown. c, d MT+/+ (n = 6) and MT-/- (n = 6) Tr1 cells were stimulated with plate-bound anti-CD3 [5 μg/mL] for 48 h. c The fold increase in intracellular [Zn2+] was measured at 4, 8, 12, 24 and 48 h. d The intracellular [Zn2+] of each sample after 48 h is shown. Error bars indicate standard deviation. (*p < .05)