Fig. 6
From: Metallothionein regulates intracellular zinc signaling during CD4+ T cell activation
Release of the MT-dependent Zn2+ pool decreases IL-10 secretion but does not affect IFN-gamma release. Tr1 induced splenocyte populations from metallothionein knockout (MT-/-) (n = 3) or wildtype control mice (MT+/+) (n = 3) were restimulated with anti-CD3 [5 μg/mL] for 48 h. Supernatants were collected at different timepoints and analyzed for the presence of (a) IL-10 and (b) IFN-gamma by ELISA. c Tr1 cells from metallothionein knockout (MT-/-) (n = 3) or wildtype control mice (MT+/+) (n = 3) were pre-incubated with ZnSO4 [20 μM] (to increase intracellular [Zn2+]) or in media alone for 24 h before being restimulated with anti-CD3 [5 μg/mL] for an additional 24 h. Error bars indicate standard deviation. (*p < .05)