Fig. 6

Involvement of recruitment factors in gastrointestinal tract plasma cells. a Quantification of MAdCAM-1 protein expression in large intestine tissue from BALB/c and ΔdblGATA-1 naïve mice (n = 7-8; combined from 2 independent experiments) and at 21 days post-infection with T. muris (n = 6-9; combined from 2 independent experiments). Representative photos of immunohistochemistry in large intestine tissue from (b); BALB/c and (c); ΔdblGATA-1 mice at 21 days post-infection with T. muris. d; Quantification of MAdCAM-1 protein expression in small intestine tissue from BALB/c and ΔdblGATA-1 naïve mice (n = 4-6; combined from 2 independent experiments) and at day 10 post infection with T. gondii PRU (n = 5-8; combined from 2 independent experiments). Representative photos of immunohistochemistry in small intestine tissue from (e); BALB/c and (f); ΔdblGATA-1 mice at 10 days post-infection with T. gondii PRU. g; CCL25 mRNA expression was assessed by Q-PCR in samples of large intestine tissue from BALB/c and ΔdblGATA-1 mice. Data shows naïve mice (n = 3-4) and at day 21 post-infection with T. muris (n = 6-8; combined from 2 independent experiments). h; CCL25 mRNA expression in small intestine tissue from BALB/c and ΔdblGATA-1 mice; naïve mice (n = 4-5) and at day 10 post-infection with T. gondii PRU (n = 8; combined from 2 independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001. Two-way ANOVA with post-hoc Bonferroni or student’s t-test as appropriate. Scale Bar represents 50 μm. i; Representative photos of immunohistochemistry in the small intestine tissue from BALB/c and ΔdblGATA-1 mice: naïve and at 10 days post-infection with T. gondii PRU. Sections are stained with IgA (red), Ki67 (green) and DAPI (blue). Scale Bar represents 100 μm