Fig. 1

Serglycin proteoglycans limits T. spiralis induced entheropathy. a Intestinal worm burden. b-h Paraffin embedded intestinal tissues from control and infected WT and SG^−/− mice (12 dpi) were sectioned, stained with H&E and the histopathological changes observed. b Shortening of the villi and (c) swelling of the villi tips were quantified by measuring the length and breadth of 15 villi per intestinal section and mouse, using the Nikon NIS software. In (d) the epithelial lesions (as defined in M&M) were counted in 10 villi per infected mouse. Representative photographs of intestinal tissues of WT and SG^−/− mice used for the scoring presented in (b, c, d) are shown in (e-h). (e) Uninfected WT, (f) uninfected SG^−/−, (g) infected WT and (h) infected SG^−/− sections of intestines at 100 X magnification. Arrows (in g and h) indicate the villi region where the length was measured. Pooled data from 3 experiments for each genotype is shown with control mice (N = 12) and infected mice (N = 15). Data is expressed as mean values (a-d) and significant differences between WT and SG^−/− mice are indicated in the figure. **P <0.001, ***P <0.0001 versus infected WT mice