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Fig. 2 | BMC Immunology

Fig. 2

From: Serglycin proteoglycans limit enteropathy in Trichinella spiralis-infected mice

Fig. 2

Serglycin proteoglycans contribute to mast cell recruitment and mast cell protease levels during T. spiralis induced enteropathy (12 dpi). Paraffin embedded intestines were sectioned and stained with CD117/c-kit antibody and Napthol AS-D chloroacetate esterase for detection and quantification of MCs. a WT and (b) SG−/− intestinal sections at 200 X magnification stained with CD117; (d) WT and (e) SG−/− sections at 100 X magnification stained with chloroacetate esterase. Arrows point to crypt MCs and arrowheads to villus MCs. c CD117 positive MC counts in 50 villus crypt units (VCUs) in infected WT and SG−/− mice. In (f) counts of chloroacetate esterase stained MCs in 25 villus and 25 crypts in infected WT and SG−/− mice are shown. In (g) intestinal levels of MCPT5 and (h) MCPT6 were analyzed by western blotting and the relative signal intensity estimated with Image J, and in (i) intestinal levels of MCPT1 was analyzed by ELISA. In c and f, pooled data from three experiments is shown with infected mice (N = 15) for each genotype. In g and h, the relative signal intensity is calculated on N = 6 for each genotype. In I, pooled data from 2 experiments is shown with infected mice (N = 12) for each genotype. Data is expressed as mean values (in c, f, i) and mean values + SEM (in g, h), and significant differences between genotypes are indicated in the figure. Not significant, ns P >0.05, ***P <0.0001 versus WT mice and ###P <0.0001 versus infected mice

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