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Fig. 2 | BMC Immunology

Fig. 2

From: Reconstitution of immune cell in liver and lymph node of adult- and newborn-engrafted humanized mice

Fig. 2

Differences in profile of human lymphoid and myeloid cell reconstitution between spleen, bone marrow, blood, and thymus. At 22 to 28 post-engraftment, spleen, bone marrow, blood, and thymus were isolated, processed, and examined for human CD45, CD3, CD4, CD8, CD56, CD14, and CD19 expression. All events were first gated on human CD45 expression and subsequently examined for T- and B-cell, NK cell, NKT cell, and myeloid cell-specific markers. Human CD45+ cells were first examined for CD3 and CD56 expression. Representative flow plots for each tissue are displayed in (a). Proportions of human CD3+ T-cells stratified by tissue shown in (b). Proportions of human CD3-CD56+ NK cells shown in (c). Proportions of human CD3 + CD56+ NKT cells graphically shown in (d). Human CD3+ cells were then examined for human CD4+ and CD8+ expression. Representative flow plots shown in (e) and proportions of human CD4+ and CD8+ T-cells within each tissue are shown in (f). Human CD45+ cells were then examined for CD19+ B-cell expression and CD14+ cell expression. Representative flow plots shown in (g) and (i) for B-cell expression and myeloid cell expression, respectively, in each tissue and graphically represented in (h) and (j). n = 3; *p < 0.05

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