Fig. 3
From: Reconstitution of immune cell in liver and lymph node of adult- and newborn-engrafted humanized mice
T- and B-cells constitute lymph nodes from human immune cell engrafted pups and adults. Lymph nodes were isolated from adult- and pup-engrafted humanized mice, processed, and examined for mouse CD45 and human CD45, CD3, CD4, CD8, CD56, CD19, and CD14 expression. Events were first analyzed for mouse and human CD45 expression. Representative flow plots displayed in (a) and graphically represented in (b). Based on human CD45 expression, lymph nodes were then analyzed for CD3, CD56, CD14, and CD19 expression. Representative flow plots shown in (c) and stratified by tissue in (d). Human CD3 cells were further examined for human CD4 and CD8 expression, shown in (e) and (f). n = 2