Fig. 5

Differential expression of human CD16, NKp30, and CD11b on NK cells in the liver, spleen, BM, blood thymus and liver of CD34+ hematopoietic stem cell-engrafted pups and adult humanized mice. Liver, spleen, bone marrow, blood, thymus, and lymph node were isolated from adult- or pup-engrafted humanized mice 22–28 weeks post-engraftment, processed, and examined for a human CD45, CD3, CD56, CD16, NKp44, NKG2D, NKp30, CD27, CD11b, and CD57. Cells were first gated on human CD45 + cells and subsequently analyzed for human CD3 and CD56 expression. NK cells (CD3-CD56+) from all isolated tissues were then analyzed for CD16, NKp44, NKG2D, and NKp30 expression in (a), (b), (c), and (d), respectively. NK cells from liver, spleen, and bone marrow were analyzed for CD27, CD11b, and CD57 expression in (e), (f), and (g), respectively. n = 5–8; *p < 0.05