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Fig. 1 | BMC Immunology

Fig. 1

From: Immunoregulatory effects triggered by immunobiotic Lactobacillus jensenii TL2937 strain involve efficient phagocytosis in porcine antigen presenting cells

Fig. 1

Phagocytosis of immunobiotic Lactobacillus jensenii TL2937 by mononuclear phagocytes from porcine Peyer’s patches (PPMPs). Adherent PPMPs were treated with the immunobiotic strain L. jensenii TL2937 or the non-immunoregulatory strain L. plantarum TL2766. Untreated adherent PPMPs were used as controls. Bacterial phagocytosis by adherent CD172a+CD11R1+ or CD172aCD11R1+ PPMPs was evaluated using flow cytometric analysis. Histograms represent data from the flow cytometric analysis as follows: cells treated with lactobacilli (solid line), and untreated control cells (dot line) (a). Isolated CD172a+ PPMPs cells were obtained by a MACS cell separation system (magnetic cell labeling). Isolated CD172a+ cells were treated with L. jensenii TL2937 or L. plantarum TL2766. Bacterial phagocytosis was evaluated using flow cytometric analysis (b). In all the experiments cells were used in a concentration of 5.0 × 106 cells/well. Values of mean fluorescence intensity (MFI) are shown for each group. The results represent data from three independent experiments using different donors

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